Application of high throughput pretreatment and co-hydrolysis system to thermochemical pretreatment. Part 1: Dilute acid

Authors

  • Xiadi Gao,

    1. Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, 446 Winston Chung Hall 900 University AveRiverside, California, 92521; telephone: 951-781-5703; fax: 951-781-5790
    2. Center for Environmental Research and Technology, Bourns College of Engineering, University of California, Riverside, 1084 Columbia Avenue, Riverside, California 92507
    3. BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831
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  • Rajeev Kumar,

    1. Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, 446 Winston Chung Hall 900 University AveRiverside, California, 92521; telephone: 951-781-5703; fax: 951-781-5790
    2. Center for Environmental Research and Technology, Bourns College of Engineering, University of California, Riverside, 1084 Columbia Avenue, Riverside, California 92507
    3. BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831
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  • Jaclyn D. DeMartini,

    1. Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, 446 Winston Chung Hall 900 University AveRiverside, California, 92521; telephone: 951-781-5703; fax: 951-781-5790
    2. Center for Environmental Research and Technology, Bourns College of Engineering, University of California, Riverside, 1084 Columbia Avenue, Riverside, California 92507
    3. BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831
    Current affiliation:
    1. DuPont Industrial Biosciences, 925 Page Mill Road, Palo Alto, CA 94304.
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  • Hongjia Li,

    1. Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, 446 Winston Chung Hall 900 University AveRiverside, California, 92521; telephone: 951-781-5703; fax: 951-781-5790
    2. Center for Environmental Research and Technology, Bourns College of Engineering, University of California, Riverside, 1084 Columbia Avenue, Riverside, California 92507
    3. BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831
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  • Charles E. Wyman

    Corresponding author
    1. Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, 446 Winston Chung Hall 900 University AveRiverside, California, 92521; telephone: 951-781-5703; fax: 951-781-5790
    2. Center for Environmental Research and Technology, Bourns College of Engineering, University of California, Riverside, 1084 Columbia Avenue, Riverside, California 92507
    3. BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831
    • Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, 446 Winston Chung Hall 900 University AveRiverside, California, 92521; telephone: 951-781-5703; fax: 951-781-5790
    Search for more papers by this author

Abstract

Because conventional approaches for evaluating sugar release from the coupled operations of pretreatment and enzymatic hydrolysis are extremely time and material intensive, high throughput (HT) pretreatment and enzymatic hydrolysis systems have become vital for screening large numbers of lignocellulosic biomass samples to identify feedstocks and/or processing conditions that significantly improve performance and lower costs. Because dilute acid pretreatment offers many important advantages in rendering biomass highly susceptible to subsequent enzymatic hydrolysis, a high throughput pretreatment and co-hydrolysis (HTPH) approach was extended to employ dilute acid as a tool to screen for enhanced performance. First, a single-step neutralization and buffering method was developed to allow effective enzymatic hydrolysis of the whole pretreated slurry. Switchgrass and poplar were then pretreated with 0.5% and 1% acid loadings at a 5% solids concentration, the resulting slurry conditioned with the buffering approach, and the entire mixture enzymatically hydrolyzed. The resulting sugar yields demonstrated that single-step neutralizing and buffering was capable of adjusting the pH as needed for enzymatic saccharification, as well as overcoming enzyme inhibition by compounds released in pretreatment. In addition, the effects of pretreatment conditions and biomass types on susceptibility of pretreated substrates to enzymatic conversion were clearly discernible, demonstrating the method to be a useful extension of HTPH systems. Biotechnol. Bioeng. 2013; 110: 754–762. © 2012 Wiley Periodicals, Inc.

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