Article

Assessing protein oxidation by inorganic nanoparticles with enzyme-linked immunosorbent assay (ELISA)†

  1. Wenjie Sun*,
  2. Antonia Luna-Velasco,
  3. Reyes Sierra-Alvarez,
  4. Jim A. Field

Article first published online: 1 NOV 2012

DOI: 10.1002/bit.24754

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 110, Issue 3, pages 694–701, March 2013

Additional Information

How to Cite

Sun, W., Luna-Velasco, A., Sierra-Alvarez, R. and Field, J. A. (2013), Assessing protein oxidation by inorganic nanoparticles with enzyme-linked immunosorbent assay (ELISA). Biotechnol. Bioeng., 110: 694–701. doi: 10.1002/bit.24754

Author Information

  1. Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, Arizona; telephone: +1-520-621 6162; fax: +1-520-621-6048

*Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, Arizona; telephone: +1-520-621 6162; fax: +1-520-621-6048

  1. †

    Additional supporting information may be found in the online version of this article.

Publication History

  1. Issue published online: 18 JAN 2013
  2. Article first published online: 1 NOV 2012
  3. Accepted manuscript online: 10 OCT 2012 06:59AM EST
  4. Manuscript Accepted: 1 OCT 2012
  5. Manuscript Revised: 6 SEP 2012
  6. Manuscript Received: 25 JUN 2012

Funded by

  • Semiconductor Research Corporation/SEMATECH Engineering Research Center for Environmentally Benign Semiconductor Manufacturing

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Keywords:

  • nanotoxicity;
  • inorganic nanoparticles;
  • protein oxidation;
  • reactive oxygen species (ROS)

Abstract

Growth in the nanotechnology industry is leading to increased production of engineered nanoparticles (NPs). This has given rise to concerns about the potential adverse and toxic effects to biological system and the environment. An important mechanism of NP toxicity is oxidative stress caused by the formation of reactive oxygen species (ROS) or via direct oxidation of biomolecules. In this study, a protein oxidation assay was developed as an indicator of biomolecule oxidation by NPs. The oxidation of the protein, bovine serum albumin (BSA) was evaluated with an enzyme-linked immunosorbent assay (ELISA) to measure the protein carbonyl derivatives formed from protein oxidation. The results showed that some NPs such as Cu(0), CuO, Mn2O3, and Fe(0) caused oxidation of BSA; whereas, many of the other NPs tested were not reactive or very slowly reactive with BSA. The mechanisms involved in the oxidation of BSA protein by the reactive NPs could be attributed to the combined effects of ROS-dependent and direct protein oxidation mechanisms. The ELISA assay is a promising method for the assessment of protein oxidation by NPs, which can provide insights on NP toxicity mechanisms. Biotechnol. Bioeng. 2013; 110: 694–701. © 2012 Wiley Periodicals, Inc.

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