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IL-17F co- ;expression improves cell growth characteristics and enhances recombinant protein production during CHO cell line engineering

Authors

  • Mathias Contie,

    1. Laboratory of Molecular Biotechnology, Institute of Biotechnology, University of Lausanne, and Center for Biotechnology UNIL-EPFL, Lausanne, Switzerland
    2. NovImmune SA, 14 Ch. des Aulx, Plan-Les-Ouates 1228, Switzerland; telephone: +41-21-839-71-41; fax: 41-21-839-71-42
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  • Olivier Leger,

    1. NovImmune SA, 14 Ch. des Aulx, Plan-Les-Ouates 1228, Switzerland; telephone: +41-21-839-71-41; fax: 41-21-839-71-42
    Current affiliation:
    1. Merck Serono SA, 9 Chemin des Mines, Geneva 1202, Switzerland.
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  • Nicolas Fouque,

    1. NovImmune SA, 14 Ch. des Aulx, Plan-Les-Ouates 1228, Switzerland; telephone: +41-21-839-71-41; fax: 41-21-839-71-42
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  • Yves Poitevin,

    1. NovImmune SA, 14 Ch. des Aulx, Plan-Les-Ouates 1228, Switzerland; telephone: +41-21-839-71-41; fax: 41-21-839-71-42
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  • Marie Kosco-Vilbois,

    1. NovImmune SA, 14 Ch. des Aulx, Plan-Les-Ouates 1228, Switzerland; telephone: +41-21-839-71-41; fax: 41-21-839-71-42
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  • Nicolas Mermod,

    1. Laboratory of Molecular Biotechnology, Institute of Biotechnology, University of Lausanne, and Center for Biotechnology UNIL-EPFL, Lausanne, Switzerland
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  • Greg Elson

    1. NovImmune SA, 14 Ch. des Aulx, Plan-Les-Ouates 1228, Switzerland; telephone: +41-21-839-71-41; fax: 41-21-839-71-42
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  • Conflicts of interest: The authors are either employed by or consultants for NovImmune SA.

Abstract

The generation of a high productivity cell line is a critical step in the production of a therapeutic protein. Many innovative engineering strategies have been devised in order to maximize the expression rate of production cells for increased process efficiency. Less effort has focused on improvements to the cell line generation process, which is typically long and laborious when using mammalian cells. Based on unexpected findings when generating stable CHO cell lines expressing human IL-17F, we studied the benefit of expressing this protein during the establishment of production cell lines. We demonstrate that IL-17F expression enhances the rate of selection and overall number of selected cell lines as well as their transgene expression levels. We also show that this benefit is observed with different parental CHO cell lines and selection systems. Furthermore, IL-17F expression improves the efficiency of cell line subcloning processes. IL-17F can therefore be exploited in a standard manufacturing process to obtain higher productivity clones in a reduced time frame. Biotechnol. Bioeng. 2013; 110: 1153–1163. © 2012 Wiley Periodicals, Inc.

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