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Bioreducible poly(amido amine)s with different branching degrees as gene delivery vectors

Authors

  • Bo Zhang,

    1. Center for Bionanoengineering and the State Key Laboratory for Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China 310027; telephone: 86-571-87953993; fax: 86-571-87953993
    2. Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming 82071
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    • Visiting student to ZJU.

  • Xinpeng Ma,

    1. Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming 82071
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  • William Murdoch,

    1. Department of Animal Science, University of Wyoming, Laramie, Wyoming 82071
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  • Maciej Radosz,

    1. Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming 82071
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  • Youqing Shen

    Corresponding author
    1. Center for Bionanoengineering and the State Key Laboratory for Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China 310027; telephone: 86-571-87953993; fax: 86-571-87953993
    2. Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming 82071
    • Center for Bionanoengineering and the State Key Laboratory for Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China 310027; telephone: 86-571-87953993; fax: 86-571-87953993
    Search for more papers by this author

Abstract

Based on the knowledge that cationic polymers with different topographical structures behave differently in gene transfection process, herein, we synthesized three biodegradable poly(amido amine)s (PAAs) with the same repeating units and molecular weights except for degree of branching: linear PAA (LPAA), low-branched PAA (LBPAA), and high-branched PAA (HBPAA). We found that LBPAA could more effectively compact pDNA into positively charged nanoparticles than both HBPAA and LPAA. LBPAA polyplexes had the highest transfection efficiency among the three PAA polyplexes, and the difference in transfection efficiency is mainly attributed to the endocytosis rate. The cytotoxicity of PAAs was negligible at the transfection doses, probably due to the degradable disulfide bonds. Therefore, we could use branching as a parameter to simply tune a polymer's cellular uptake behavior and transfection efficiency. Biotechnol. Bioeng. 2013; 110: 990–998. © 2012 Wiley Periodicals, Inc.

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