Non-invasive online detection of microbial lysine formation in stirred tank bioreactors by using calorespirometry
Article first published online: 17 JAN 2013
Copyright © 2012 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 110, Issue 5, pages 1386–1395, May 2013
How to Cite
Regestein, L., Maskow, T., Tack, A., Knabben, I., Wunderlich, M., Lerchner, J. and Büchs, J. (2013), Non-invasive online detection of microbial lysine formation in stirred tank bioreactors by using calorespirometry. Biotechnol. Bioeng., 110: 1386–1395. doi: 10.1002/bit.24815
- Issue published online: 20 MAR 2013
- Article first published online: 17 JAN 2013
- Accepted manuscript online: 27 DEC 2012 02:11PM EST
- Manuscript Accepted: 11 DEC 2012
- Manuscript Revised: 26 NOV 2012
- Manuscript Received: 7 SEP 2012
- Corynebacterium glutamicum;
- stirred tank reactor
Non-invasive methods for online monitoring of biotechnological processes without compromising the integrity of the reactor system are very important to generate continuous data. Even though calorimetry has been used in conventional biochemical analysis for decades, it has not yet been specifically applied for online detection of product formation at technical scale. Thus, this article demonstrates a calorespirometric method for online detection of microbial lysine formation in stirred tank bioreactors. The respective heat generation of two bacterial strains, Corynebacterium glutamicum ATCC 13032 (wild-type) and C. glutamicum DM1730 (lysine producer), was compared with the O2-consumption in order to determine whether lysine was formed. As validation of the proposed calorespirometric method, the online results agreed well with the offline measured data. This study has proven that calorespirometry is a viable non-invasive technique to detect product formation at any time point. Biotechnol. Bioeng. 2013; 110: 1386–1395. © 2012 Wiley Periodicals, Inc.