Fang Zhang and Yan Zhang contributed equally to this work.
A modified metabolic model for mixed culture fermentation with energy conserving electron bifurcation reaction and metabolite transport energy†
Article first published online: 22 FEB 2013
Copyright © 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 110, Issue 7, pages 1884–1894, July 2013
How to Cite
Zhang, F., Zhang, Y., Chen, M., van Loosdrecht, M. C.M. and Zeng, R. J. (2013), A modified metabolic model for mixed culture fermentation with energy conserving electron bifurcation reaction and metabolite transport energy. Biotechnol. Bioeng., 110: 1884–1894. doi: 10.1002/bit.24855
- Issue published online: 25 MAY 2013
- Article first published online: 22 FEB 2013
- Accepted manuscript online: 4 FEB 2013 08:02AM EST
- Manuscript Accepted: 25 JAN 2013
- Manuscript Revised: 14 JAN 2013
- Manuscript Received: 4 NOV 2012
- Natural Science Foundation of China. Grant Number: 50978244
- Hundred-Talent Program of CAS
- Fundamental Research Funds for the Central Universities. Grant Number: WK2060190007
- metabolic modeling;
- variable stoichiometry;
- mixed culture fermentation;
- energy conserving electron bifurcation reaction;
- metabolite transport energy;
- transport rate coefficient
A modified metabolic model for mixed culture fermentation (MCF) is proposed with the consideration of an energy conserving electron bifurcation reaction and the transport energy of metabolites. The production of H2 related to NADH/NAD+ and Fdred/Fdox is proposed to be divided in three processes in view of energy conserving electron bifurcation reaction. This assumption could fine-tune the intracellular redox balance and regulate the distribution of metabolites. With respect to metabolite transport energy, the proton motive force is considered to be constant, while the transport rate coefficient is proposed to be proportional to the octanol–water partition coefficient. The modeling results for a glucose fermentation in a continuous stirred tank reactor show that the metabolite distribution is consistent with the literature: (1) acetate, butyrate, and ethanol are main products at acidic pH, while the production shifts to acetate and propionate at neutral and alkali pH; (2) the main products acetate, ethanol, and butyrate shift to ethanol at higher glucose concentration; (3) the changes for acetate and butyrate are following an increasing hydrogen partial pressure. The findings demonstrate that our modified model is more realistic than previous proposed model concepts. It also indicates that inclusion of an energy conserving electron bifurcation reaction and metabolite transport energy for MCF is sound in the viewpoint of biochemistry and physiology. Biotechnol. Bioeng. 2013; 110: 1884–1894. © 2013 Wiley Periodicals, Inc.