Reducing infectivity of HIV upon exposure to surfaces coated with N,N-dodecyl, methyl-polyethylenimine

Authors

  • Stephen E. Gerrard,

    Corresponding author
    1. Department of Chemical Engineering and Biotechnology, BioScience Engineering Research Group, University of Cambridge, New Museums Site, Pembroke Street, Cambridge CB2 3RA, UK; telephone: +44-1223-763969; fax: +44-1223-334796
    2. Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, California
    3. Division of Epidemiology, School of Public Health, University of California, Berkeley, California
    • Department of Chemical Engineering and Biotechnology, BioScience Engineering Research Group, University of Cambridge, New Museums Site, Pembroke Street, Cambridge CB2 3RA, UK; telephone: +44-1223-763969; fax: +44-1223-334796
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  • Alyssa M. Larson,

    1. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts
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  • Alexander M. Klibanov,

    1. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts
    2. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts
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  • Nigel K.H. Slater,

    1. Department of Chemical Engineering and Biotechnology, BioScience Engineering Research Group, University of Cambridge, New Museums Site, Pembroke Street, Cambridge CB2 3RA, UK; telephone: +44-1223-763969; fax: +44-1223-334796
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  • Carl V. Hanson,

    1. Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, California
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  • Barbara F. Abrams,

    1. Division of Epidemiology, School of Public Health, University of California, Berkeley, California
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  • Mary K. Morris

    1. Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, California
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Abstract

The infectivity of high-titer, cell-free HIV in culture media and human milk is rapidly reduced upon exposure to polyethylene slides painted with the linear hydrophobic polycation N,N-dodecyl,methyl-polyethylenimine (DMPEI). Accompanying viral p24 protein and free viral RNA analysis of solutions exposed to DMPEI-coated surfaces suggests that virion attachment to the polycationic surface and its subsequent inactivation are the likely mechanism of this phenomenon. Biotechnol. Bioeng. 2013; 110: 2058–2062. © 2013 Wiley Periodicals, Inc.

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