Enzyme production by the mixed fungal culture with nano-shear pretreated biomass and lignocellulose hydrolysis

Authors

  • Jue Lu,

    Corresponding author
    1. Technova Corporation, 1926 Turner Street, Lansing, Michigan 48906; telephone: 1-517-485-1402; fax: 1-517-485-1402
    • Technova Corporation, 1926 Turner Street, Lansing, Michigan 48906; telephone: 1-517-485-1402; fax: 1-517-485-1402.
    Search for more papers by this author
  • Rankothge Ranjith Weerasiri,

    1. Technova Corporation, 1926 Turner Street, Lansing, Michigan 48906; telephone: 1-517-485-1402; fax: 1-517-485-1402
    Search for more papers by this author
  • Yan Liu,

    1. Department of Biosystem Engineering, Michigan State University, East Lansing, Michigan
    Search for more papers by this author
  • Wei Wang,

    1. Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan 48824-1226; telephone: 1-517-355-9291; fax: 1-517-432-1105
    Search for more papers by this author
  • Shaowen Ji,

    1. Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan 48824-1226; telephone: 1-517-355-9291; fax: 1-517-432-1105
    Search for more papers by this author
  • Ilsoon Lee

    Corresponding author
    1. Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan 48824-1226; telephone: 1-517-355-9291; fax: 1-517-432-1105
    • Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan 48824-1226; telephone: 1-517-355-9291; fax: 1-517-432-1105.
    Search for more papers by this author

Abstract

Cellulase, xylanase, and β-glucosidase production was studied on novel nano-shear pretreated corn stover by the mixed fungi culture. The high shear force from a modified Tayor-Couette nano-shear mixing reactor efficiently disintegrated corn stover, resulting in a homogeneous watery mash with particles in much reduced size. Scanning electron microscope study showed visible mini-pores on the fiber cell wall surface, which could improve the accessibility of the pretreated corn stover to microorganisms. Mixed fungal culture of Trichoderma reesei RUT-C30 and Aspergillus niger produced enzymes with higher cellulolytic and xylanolytic activities on corn stover pretreated with nano-shear mixing reactor, in comparison with other pretreatment methods, including acid and ammonia fiber explosion (AFEX) pretreatment. The hydrolytic potential of the whole fermentation broth from the mixed fungi was studied, and the possibility of applying the whole cell saccharification concept was also investigated to further reduce the cost of lignocellulose hydrolysis. Biotechnol. Bioeng. 2013; 110: 2123–2130. © 2013 Wiley Periodicals, Inc.

Ancillary