Conflict of interest statement: None declared.
Transcription activator-like effector nucleases (TALENs): A highly efficient and versatile tool for genome editing†
Article first published online: 7 APR 2013
Copyright © 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 110, Issue 7, pages 1811–1821, July 2013
How to Cite
Sun, N. and Zhao, H. (2013), Transcription activator-like effector nucleases (TALENs): A highly efficient and versatile tool for genome editing. Biotechnol. Bioeng., 110: 1811–1821. doi: 10.1002/bit.24890
- Issue published online: 25 MAY 2013
- Article first published online: 7 APR 2013
- Accepted manuscript online: 18 MAR 2013 09:51AM EST
- Manuscript Accepted: 26 FEB 2013
- Manuscript Revised: 18 FEB 2013
- Manuscript Received: 21 JAN 2013
- National Institutes of Health. Grant Number: R21 HL089418
- Department of Chemical and Biomolecular Engineering at the University of Illinois at Urbana-Champaign
- double-strand break;
- genome engineering;
- gene targeting;
- homologous recombination;
- non-homologous end joining
Transcription activator-like effector (TALE) nucleases (TALENs) have recently emerged as a revolutionary genome editing tool in many different organisms and cell types. The site-specific chromosomal double-strand breaks introduced by TALENs significantly increase the efficiency of genomic modification. The modular nature of the TALE central repeat domains enables researchers to tailor DNA recognition specificity with ease and target essentially any desired DNA sequence. Here, we comprehensively review the development of TALEN technology in terms of scaffold optimization, DNA recognition, and repeat array assembly. In addition, we provide some perspectives on the future development of this technology. Biotechnol. Bioeng. 2013; 110: 1811–1821. © 2013 Wiley Periodicals, Inc.