Transcription activator-like effector nucleases (TALENs): A highly efficient and versatile tool for genome editing

Authors

  • Ning Sun,

    1. Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801
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  • Huimin Zhao

    Corresponding author
    1. Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801
    2. Department of Chemical and Biomolecular Engineering, Department of Bioengineering, Department of Chemistry, Center for Biophysics and Computational Biology, and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801; telephone: 217-333-2631; fax: 217-333-5052
    • Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801
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  • Conflict of interest statement: None declared.

Abstract

Transcription activator-like effector (TALE) nucleases (TALENs) have recently emerged as a revolutionary genome editing tool in many different organisms and cell types. The site-specific chromosomal double-strand breaks introduced by TALENs significantly increase the efficiency of genomic modification. The modular nature of the TALE central repeat domains enables researchers to tailor DNA recognition specificity with ease and target essentially any desired DNA sequence. Here, we comprehensively review the development of TALEN technology in terms of scaffold optimization, DNA recognition, and repeat array assembly. In addition, we provide some perspectives on the future development of this technology. Biotechnol. Bioeng. 2013; 110: 1811–1821. © 2013 Wiley Periodicals, Inc.

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