Candida bombicola as a platform organism for the production of tailor-made biomolecules

Authors

  • Sophie L.K.W. Roelants,

    Corresponding author
    • Faculty of Bioscience Engineering, Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Ghent University, 9000 Ghent, Belgium
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    • Sophie L.K.W. Roelants and Karen M.J. Saerens share first authorship.
  • Karen M.J. Saerens,

    1. Faculty of Bioscience Engineering, Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Ghent University, 9000 Ghent, Belgium
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    • Sophie L.K.W. Roelants and Karen M.J. Saerens share first authorship.
  • Thibaut Derycke,

    1. Faculty of Bioscience Engineering, Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Ghent University, 9000 Ghent, Belgium
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  • Bing Li,

    1. Department of Plant Biotechnology and Bioinformatics, Ghent University, Zwijnaarde
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  • Yao-Cheng Lin,

    1. Department of Plant Systems Biology, VIB, Zwijnaarde
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  • Yves Van de Peer,

    1. Department of Plant Biotechnology and Bioinformatics, Ghent University, Zwijnaarde
    2. Department of Plant Systems Biology, VIB, Zwijnaarde
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  • Sofie L. De Maeseneire,

    1. Faculty of Bioscience Engineering, Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Ghent University, 9000 Ghent, Belgium
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  • Inge N.A. Van Bogaert,

    1. Faculty of Bioscience Engineering, Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Ghent University, 9000 Ghent, Belgium
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  • Wim Soetaert

    1. Faculty of Bioscience Engineering, Centre for Industrial Biotechnology and Biocatalysis (InBio.be), Ghent University, 9000 Ghent, Belgium
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Correspondence to: S.L.K.W. Roelants

telephone: +32-0-9-2649385

fax: +32-0-9-2646231

e-mail: sophie.roelants@ugent.be

Abstract

The yeast Candida bombicola is capable of producing high amounts (400 g/L) of the biosurfactant sophorolipids. The genetic makeup of this industrially important yeast has recently been uncovered and molecular manipulation techniques have been developed. Hence, all tools for the development of new bioprocesses with C. bombicola are now available. As a proof of concept, the production of two totally different molecules was aimed for: the bioplastic polyhydroxyalkanoate (PHA) and a new-to-nature cellobioselipid-biosurfactant. Integration of the new functionalities at genomic loci necessary for sophorolipid production safeguards the new biomolecules from sophorolipid contamination, while taking advantage of the regulation of the sophorolipid gene cluster. A maximum yield of 2.0% wt/dwt PHA was obtained; furthermore, this is the first time cellobioselipid synthesis by a non-natural producer is reported. We here provided proof of concept that C. bombicola can be transformed into a platform organism for the production of tailor-made biomolecules. Biotechnol. Bioeng. 2013; 110:2494–2503. © 2013 Wiley Periodicals, Inc.

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