Production of non-proteinogenic amino acids from α-keto acid precursors with recombinant Corynebacterium glutamicum
Version of Record online: 4 JUN 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 110, Issue 11, pages 2846–2855, November 2013
How to Cite
Kim, J.-Y., Lee, Y.-A., Wittmann, C. and Park, J.-B. (2013), Production of non-proteinogenic amino acids from α-keto acid precursors with recombinant Corynebacterium glutamicum. Biotechnol. Bioeng., 110: 2846–2855. doi: 10.1002/bit.24962
- Issue online: 21 SEP 2013
- Version of Record online: 4 JUN 2013
- Manuscript Accepted: 8 MAY 2013
- Manuscript Revised: 29 APR 2013
- Manuscript Received: 5 OCT 2012
- Seoul R&BD Program. Grant Number: KU080657
- Ministry of Land, Transport and Marine Affairs, Korea
Additional supporting information may be found in the online version of this article at the publisher's web-site.
|bit24962-sm-0001-SuppData.docx||151K||Figure S1. The SDS–PAGE analysis of BCAT expressed in the recombinant C. glutamicum pEKEx2-BCAT. The BCAT band is indicated by an arrow. The samples were prepared 4 h after induction with 0 (lanes 1, 2), 0.1 (lanes 3, 4), 0.5 (lanes 5, 6), and 1.0 (lanes 7, 8) mM IPTG. M, marker protein; lanes 1, 3, 5, 7, soluble fraction; lanes 2, 4, 6, 8, insoluble fraction. The SDS–PAGE analysis was carried out according to the method previously described (Sambrook and Russell, 2001).|
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