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bit24962-sm-0001-SuppData.docx151KFigure S1. The SDS–PAGE analysis of BCAT expressed in the recombinant C. glutamicum pEKEx2-BCAT. The BCAT band is indicated by an arrow. The samples were prepared 4 h after induction with 0 (lanes 1, 2), 0.1 (lanes 3, 4), 0.5 (lanes 5, 6), and 1.0 (lanes 7, 8) mM IPTG. M, marker protein; lanes 1, 3, 5, 7, soluble fraction; lanes 2, 4, 6, 8, insoluble fraction. The SDS–PAGE analysis was carried out according to the method previously described (Sambrook and Russell, 2001).

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