Conflict of interest: None.
Recreating a human trabecular meshwork outflow system on microfabricated porous structures
Version of Record online: 29 JUN 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 110, Issue 12, pages 3205–3218, December 2013
How to Cite
Torrejon, K. Y., Pu, D., Bergkvist, M., Danias, J., Sharfstein, S. T. and Xie, Y. (2013), Recreating a human trabecular meshwork outflow system on microfabricated porous structures. Biotechnol. Bioeng., 110: 3205–3218. doi: 10.1002/bit.24977
- Issue online: 23 OCT 2013
- Version of Record online: 29 JUN 2013
- Accepted manuscript online: 15 JUN 2013 04:17AM EST
- Manuscript Accepted: 7 JUN 2013
- Manuscript Revised: 4 JUN 2013
- Manuscript Received: 16 MAR 2013
- College of Nanoscale Science and Engineering Startup Funds
- NIH. Grant Number: R01 EY20670
- American Glaucoma Society Mid-Career award
- Department of Ophthalmology SUNY Downstate by Research to Prevent Blindness Inc
- NSF. Grant Number: CBET 0846270
- NSF Graduate Research Fellowship Program
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. SEM micrographs of HTM cells grown on glass coverslips (A) and on SU-8 scaffolds coated with poly-L-lysine (B) and gelatin (C) for 10 days. Coverslip (D), SU-8 scaffold coated with poly-L-lysine (E) and gelatin (F) with no cells.
Figure S2. (A) SEM micrograph of bioengineered HTM complex after Lat-B perfusion. (B) Three dimensional confocal reconstruction of HTM cells grown on SU-8 scaffold after Lat-B perfusion. DAPI stained nuclei in blue (top), F-actin expression in green (middle), and merged images (bottom). The SU-8 scaffold exhibits autofluorescence and is indicated by the dashed-line rectangles.
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