Rathi Saravanan and Xiang Li contributed equally to this work.
Design of short membrane selective antimicrobial peptides containing tryptophan and arginine residues for improved activity, salt-resistance, and biocompatibility
Article first published online: 5 AUG 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 1, pages 37–49, January 2014
How to Cite
Saravanan, R., Li, X., Lim, K., Mohanram, H., Peng, L., Mishra, B., Basu, A., Lee, J.-M., Bhattacharjya, S. and Leong, S. S. J. (2014), Design of short membrane selective antimicrobial peptides containing tryptophan and arginine residues for improved activity, salt-resistance, and biocompatibility. Biotechnol. Bioeng., 111: 37–49. doi: 10.1002/bit.25003
- Issue published online: 22 NOV 2013
- Article first published online: 5 AUG 2013
- Accepted manuscript online: 16 JUL 2013 07:57AM EST
- Manuscript Accepted: 8 JUL 2013
- Manuscript Revised: 30 MAY 2013
- Manuscript Received: 28 MAR 2013
- Biomedical Engineering Program, A*Star. Grant Number: BEP 103149001
- INSIST initiative (NTU)
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. Secondary structure analysis of the amidated decamers in solution and upon interaction with lipid micelles. Far UV circular dichroism spectra of the peptides in 10 mM phosphate buffer, pH 7.2 (panel A), negatively charged SDS (panel B), zwitterionic DPC micelles (panel C), and DPC bound D5-NH2 and D6-NH2 in the presence and absence of salt (panel D) recorded at 25°C.
Figure S2. Overlay of the selected sections of the 2D NOESY spectra of D5-NH2 (panel A) and D6-NH2 (panel B) in 10 mM sodium phosphate buffer (cyan contour) and in solution containing 200 mM SDS (red contour) showing NOE correlations among down field shifted amide and aromatic resonances (along ω2) with upfield shifted resonances (along ω1).
Table SI. Summary of the structural statistics of 20 lowest energy structures of D5 and D6 in SDS micelles calculated using CYANA.
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