This article was published online on 6 September 2013. Subsequently, it was determined that an error had been made in the Acknowledgments, and the correction was published on 13 September 2013.
Cell-free protein expression based on extracts from CHO cells
Article first published online: 6 SEP 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 1, pages 25–36, January 2014
How to Cite
Brödel, A. K., Sonnabend, A. and Kubick, S. (2014), Cell-free protein expression based on extracts from CHO cells. Biotechnol. Bioeng., 111: 25–36. doi: 10.1002/bit.25013
- Issue published online: 22 NOV 2013
- Article first published online: 6 SEP 2013
- Accepted manuscript online: 30 AUG 2013 06:01AM EST
- Manuscript Accepted: 15 JUL 2013
- Manuscript Revised: 8 JUL 2013
- Manuscript Received: 17 APR 2013
- German Ministry of Education and Research. Grant Number: 0312039
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. cDNA of CrPV IGR IRES (GenBank accession no. AF218039, nucleotides 6,025–6,216) harboring either ATG (A) or GCT (B) as initiation codon (underlined).
Figure S2. Design of the expression vector EasyXpress pIX3.0 CrPV IGR IRES (A) and pcDNA3.1(+) CrPV IGR IRES (B). The pcDNA3.1 CrPV IGR IRES vector contains a CMV and a T7 promoter whereas the EasyXpress pIX3.0 CrPV IGR IRES construct is limited to the T7 promoter. As a result of the limitation, the use of the pIX3.0-based vector is restricted to protein expression in vitro but enables increased protein expression levels in the CHO-based cell-free protein production system. Different expression levels may be attributed to the different transcriptional products encoding the target protein.
TABLE SI. Oligonucleotide sequences used for DNA template design.
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