Engineering of Saccharomyces cerevisiae for the synthesis of short chain fatty acids
Version of Record online: 3 SEP 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 2, pages 347–358, February 2014
How to Cite
Leber, C. and Da Silva, N. A. (2014), Engineering of Saccharomyces cerevisiae for the synthesis of short chain fatty acids. Biotechnol. Bioeng., 111: 347–358. doi: 10.1002/bit.25021
- Issue online: 19 DEC 2013
- Version of Record online: 3 SEP 2013
- Accepted manuscript online: 8 AUG 2013 07:53AM EST
- Manuscript Accepted: 2 AUG 2013
- Manuscript Revised: 19 JUL 2013
- Manuscript Received: 6 MAY 2013
- National Science Foundation. Grant Number: EEC-0813570
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. Confirmation of active short chain thioesterases expressed in yeast. In vitro DTNB assays demonstrated activity of short chain thioesterases CpFatB1 (strain BY4741 expressing pXP842-CpFatB1) () and TEII (strain BY4741 expressing pXP842-TEII) () on short chain carbon substrates C8-CoA (solid line), C10-CoA (dashed line), and C12-CoA (dotted line). Specific activities of CpFatB1 on caprylic acid-CoA, capric acid-CoA, and lauric acid-CoA are 40.0, 54.0, and 43.3 nmol min−1 mg−1, respectively. Specific activities of TEII on caprylic acid-CoA, capric acid-CoA, and lauric acid-CoA are 28.0, 7.5, and 4.7 nmol min−1 mg−1, respectively.
Table SI. List and sequence of PCR primers.
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