The clinical and commercial use of pluripotent cells is highly reliant on the development of tightly controlled culture processes. The Szita group combines microfluidic approaches with analytical methods to create novel tools that enhance our understanding of these processes. In this work, the authors developed a novel method for adherent cell culture monitoring which combines a widely used light microscopy method with automated image processing to monitor key culture characteristics such as confluency, cell density, and morphology.
The method described in this study was designed from the ground-up to meet the requirements for routine use in cell culture laboratories. Its simplicity and short measurement times rival those of visual inspection by operators while generating robust quantitative data suitable for process documentation or evaluation of experimental outcomes. Applications to a wide variety of cell behavior monitoring (including proliferation, cell death, and transient morphological changes) are also featured in this article.