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Figure S1. Comparison of acetone and the 50 kDa dextran elution peaks in the protein-immobilized column.

Figure S2. Retention volume (bottom panel) and second virial coefficient (upper panel) as a function of ionic strength for the monoclonal antibody in 5 mM acetate buffer at pH 5. The solid curve in the upper panel is a model calculation for second virial coefficients developed using the potential of mean force for charge–charge electrostatic interactions between hard spheres.

Table S1. Retention volumes for the dextran standards and the monoclonal antibody determined from size-exclusion chromatography using the Superdex column.

Table S2. Comparison of second virial coefficients determined by self-interaction chromatography and membrane osmometery.

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