The osmotic pressure of highly concentrated monoclonal antibody solutions: Effect of solution conditions
Article first published online: 16 SEP 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 3, pages 529–536, March 2014
How to Cite
Binabaji, E., Rao, S. and Zydney, A. L. (2014), The osmotic pressure of highly concentrated monoclonal antibody solutions: Effect of solution conditions. Biotechnol. Bioeng., 111: 529–536. doi: 10.1002/bit.25104
- Issue published online: 21 JAN 2014
- Article first published online: 16 SEP 2013
- Accepted manuscript online: 31 AUG 2013 06:31AM EST
- Manuscript Accepted: 21 AUG 2013
- Manuscript Revised: 14 AUG 2013
- Manuscript Received: 9 JUN 2013
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. Comparison of acetone and the 50 kDa dextran elution peaks in the protein-immobilized column.
Figure S2. Retention volume (bottom panel) and second virial coefficient (upper panel) as a function of ionic strength for the monoclonal antibody in 5 mM acetate buffer at pH 5. The solid curve in the upper panel is a model calculation for second virial coefficients developed using the potential of mean force for charge–charge electrostatic interactions between hard spheres.
Table S1. Retention volumes for the dextran standards and the monoclonal antibody determined from size-exclusion chromatography using the Superdex column.
Table S2. Comparison of second virial coefficients determined by self-interaction chromatography and membrane osmometery.
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