Nandita Vishwanathan, Huong Le, Nitya M. Jacob, and Yung-Shyeng Tsao contributed equally to this work.
Transcriptome dynamics of transgene amplification in Chinese hamster ovary cells
Version of Record online: 18 OCT 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 3, pages 518–528, March 2014
How to Cite
Vishwanathan, N., Le, H., Jacob, N. M., Tsao, Y.-S., Ng, S.-W., Loo, B., Liu, Z., Kantardjieff, A. and Hu, W.-S. (2014), Transcriptome dynamics of transgene amplification in Chinese hamster ovary cells. Biotechnol. Bioeng., 111: 518–528. doi: 10.1002/bit.25117
- Issue online: 21 JAN 2014
- Version of Record online: 18 OCT 2013
- Accepted manuscript online: 21 SEP 2013 08:07AM EST
- Manuscript Accepted: 9 SEP 2013
- Manuscript Revised: 16 AUG 2013
- Manuscript Received: 29 MAY 2013
- Vietnam Education Foundation
- NIH Biotechnology Training Grant. Grant Number: GM08347
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. The division of high and low producing clones for comparative analysis is shown. A: Titer and (B) specific productivity of the subclones cultured in fedbatch mode. The dark bars correspond to the sub-clones that are classified as high producers, the white bars correspond to the sub-clones classified as low producers and the gray bars are sub-clones that were mid-producers.
Figure S2. Scatter plot showing correlation between specific productivity and (A) specific growth rate in growth phase, (B) specific growth rate in death phase and (C) peak cell concentration in fedbatch culture for high-, low- and mid-producers. The () symbols correspond to the sub-clones that are classified as high producers, the () symbols correspond to the sub-clones classified as low producers and () symbols are sub-clones that were mid-producers.
Table SI. List of primers for qRT-PCR analysis.
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