Combinatorial engineering of mevalonate pathway for improved amorpha-4,11-diene production in budding yeast
Article first published online: 22 OCT 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 3, pages 608–617, March 2014
How to Cite
Yuan, J. and Ching, C. B. (2014), Combinatorial engineering of mevalonate pathway for improved amorpha-4,11-diene production in budding yeast. Biotechnol. Bioeng., 111: 608–617. doi: 10.1002/bit.25123
- Issue published online: 21 JAN 2014
- Article first published online: 22 OCT 2013
- Accepted manuscript online: 7 OCT 2013 01:51AM EST
- Manuscript Accepted: 23 SEP 2013
- Manuscript Revised: 8 SEP 2013
- Manuscript Received: 6 AUG 2013
- National University of Singapore
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. Gel image of PCR validation for genome integration events of mevalonate pathway genes. Lane 1 was loaded with PCR products from the parental strain S. cerevisiae harboring YB/I. Lane 2–9 were loaded with PCR products from mutant strains (1–8) with intense orange color. One kilobase DNA ladder (New England Biolabs) was loaded for estimating the band sizes.
Figure S2. Gel image of PCR validation for genome integration events of mevalonate pathway genes. PCR productsfrom five large colonies (Lanes 1–5) and three small ones (Lane 6–8) were loaded. One kilobase DNA ladder (New England Biolabs) was loaded for estimating the band sizes.
Figure S3. Melting curve profile of qPCR results showed unique amplicon peak for each reaction without primer dimer peak.
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