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Figure S1. SDS–PAGE of the proteins from E. coli ARP. Lane 1: Protein marker; lane 2: proteins from E. coli ARP.

Figure S2. The GC analysis results confirmed the production of prenol and isoprenol authentic prenol and isoprenol are purchased from Sigma-Aldrich.

Figure S3. The curves of cell growth, glucose consumption and CoQ10 accumulation of RspMCS, RspSRID, RspSRIDL, RspSRIDbL, RspSRIDcL, RspSRIDdL, and RspSRIDeL. Data are shown as means ± SD from three independent experiments.

Figure S4. The curves of cell growth, glucose consumption, and CoQ10 accumulation of RspMCS, RspSRIDpG, RspSRIDaLpG, RspSRIDbLpG, RspSRIDcLpG, RspSRIDdLpG, and RspSRIDeLpG. Data are shown as means ± SD from three independent experiments.

Table SI. Plasmids and bacterial strains used in this study.

Table SII. Primers and RBS sequences.

Table SIII. Cloning and expression of ADP-ribose pyrophosphatase.

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