Huiqing Chong and Hefang Geng have contributed equally to this paper.
Enhancing E. coli isobutanol tolerance through engineering its global transcription factor cAMP receptor protein (CRP)
Version of Record online: 6 NOV 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 4, pages 700–708, April 2014
How to Cite
Chong, H., Geng, H., Zhang, H., Song, H., Huang, L. and Jiang, R. (2014), Enhancing E. coli isobutanol tolerance through engineering its global transcription factor cAMP receptor protein (CRP). Biotechnol. Bioeng., 111: 700–708. doi: 10.1002/bit.25134
- Issue online: 22 FEB 2014
- Version of Record online: 6 NOV 2013
- Accepted manuscript online: 12 OCT 2013 04:31AM EST
- Manuscript Accepted: 10 OCT 2013
- Manuscript Revised: 16 SEP 2013
- Manuscript Received: 15 MAY 2013
- Ministry of Education, Singapore. Grant Number: MOE2012-T2-2-117
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1: DNA binding properties of IB2 CRP and native CRP with Class I, II, and III CRP-dependent promoters. The DNA binding was quantified by measuring β-galactosidase activity from reporter gene assay.
Figure S2: Glutamate decarboxylase (GAD) activity of IB2 and the control in the absence or presence of 1% isobutanol stress.
Table SI: Primers used in RT-PCR.
Table SII: Genes significantly changed (log2 fold change >2, P-value <0.05) in IB2 compared with the control under no stress.
Table SIII: Genes significantly changed (log2 fold change >2, P-value <0.05) in IB2 compared with the control with 1% isobutanol treatment.
Table SIV: Microarray and RT-PCR results of IB2 in the presence of 1% isobutanol (v/v) compared with the control.
Table SV: Microarray and RT-PCR results of IB2 in the absence of isobutanol stress compared with the control.
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