RY and KH contributed equally to this work.
Microfluidic perfusion culture of human induced pluripotent stem cells under fully defined culture conditions
Version of Record online: 30 NOV 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 5, pages 937–947, May 2014
How to Cite
Yoshimitsu, R., Hattori, K., Sugiura, S., Kondo, Y., Yamada, R., Tachikawa, S., Satoh, T., Kurisaki, A., Ohnuma, K., Asashima, M. and Kanamori, T. (2014), Microfluidic perfusion culture of human induced pluripotent stem cells under fully defined culture conditions. Biotechnol. Bioeng., 111: 937–947. doi: 10.1002/bit.25150
The authors have no conflict of interest to declare.
Ryosuke Yoshimitsu and Koji Hattori contributed equally to this work.
- Issue online: 26 MAR 2014
- Version of Record online: 30 NOV 2013
- Accepted manuscript online: 13 NOV 2013 01:44AM EST
- Manuscript Accepted: 8 NOV 2013
- Manuscript Revised: 9 OCT 2013
- Manuscript Received: 20 JUL 2013
- The Japan Science and Technology Agency
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Table SI Antibodies.
Figure S1: Effect of coating the PDMS surfacewith 10-times concentrated ECM on culturing hiPSCs indefined culture medium.
Figure S2: BMP4-induced differentiation of hiPSCs into trophoectoderm.
Figure S3: Differentiation of hiPSCs in a 96-well plate.
Figure S4: Differentiation of a different hiPSC line, Tic, in microchamber array chip.
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