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Additional supporting information may be found in the online version of this article at the publisher's web-site.

FilenameFormatSizeDescription
bit25155-sm-0001-SuppFig-S1.tif107KFigure S1 Pathway diagrams.
bit25155-sm-0002-SuppFig-S2.tif172KFigure S2 Time-course of phenotypic and global transcriptional responses to individual amino-acid withdrawal in cell line A and RT-qPCR of 24-h timepoint.
bit25155-sm-0003-SuppFig-S3.tif117KFigure S3 Effects of individual amino-acid withdrawal genes involved in cellular lipid homeostasis A.
bit25155-sm-0004-SuppFig-S4.tif178KFigure S4 Growth, titer, and amino-acid utilization profiles for antibody-producing cell lines B and C.
bit25155-sm-0005-SuppFig-S5.tif267KFigure S5 Comparison of transcriptional changes in cholesterol biosynthesis pathway effectors in response to individual amino-acid withdrawal among five antibody-producing (A–C) and parental (CS9 and CHO-K1) CHO cell lines.
bit25155-sm-0006-SuppTab-S1.docx30KTable SI Sequences of CHO-specific primers used for RT-qPCR analysis of gene expression.
bit25155-sm-0007-SuppTab-S2.docx21KTable SII Complete amino-acid utilization profile for antibody-producing cell line A.

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