Cellular responses to individual amino-acid depletion in antibody-expressing and parental CHO cell lines
Article first published online: 11 DEC 2013
© 2013 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 5, pages 965–979, May 2014
How to Cite
Fomina-Yadlin, D., Gosink, J. J., McCoy, R., Follstad, B., Morris, A., Russell, C. B. and McGrew, J. T. (2014), Cellular responses to individual amino-acid depletion in antibody-expressing and parental CHO cell lines. Biotechnol. Bioeng., 111: 965–979. doi: 10.1002/bit.25155
- Issue published online: 26 MAR 2014
- Article first published online: 11 DEC 2013
- Accepted manuscript online: 19 NOV 2013 08:25AM EST
- Manuscript Accepted: 12 NOV 2013
- Manuscript Revised: 10 OCT 2013
- Manuscript Received: 22 JUL 2013
Additional supporting information may be found in the online version of this article at the publisher's web-site.
|bit25155-sm-0001-SuppFig-S1.tif||107K||Figure S1 Pathway diagrams.|
|bit25155-sm-0002-SuppFig-S2.tif||172K||Figure S2 Time-course of phenotypic and global transcriptional responses to individual amino-acid withdrawal in cell line A and RT-qPCR of 24-h timepoint.|
|bit25155-sm-0003-SuppFig-S3.tif||117K||Figure S3 Effects of individual amino-acid withdrawal genes involved in cellular lipid homeostasis A.|
|bit25155-sm-0004-SuppFig-S4.tif||178K||Figure S4 Growth, titer, and amino-acid utilization profiles for antibody-producing cell lines B and C.|
|bit25155-sm-0005-SuppFig-S5.tif||267K||Figure S5 Comparison of transcriptional changes in cholesterol biosynthesis pathway effectors in response to individual amino-acid withdrawal among five antibody-producing (A–C) and parental (CS9 and CHO-K1) CHO cell lines.|
|bit25155-sm-0006-SuppTab-S1.docx||30K||Table SI Sequences of CHO-specific primers used for RT-qPCR analysis of gene expression.|
|bit25155-sm-0007-SuppTab-S2.docx||21K||Table SII Complete amino-acid utilization profile for antibody-producing cell line A.|
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