The authors have no conflict of interest to declare.
Optimization of liquid overlay technique to formulate heterogenic 3D co-cultures models
Article first published online: 25 FEB 2014
© 2014 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 111, Issue 8, pages 1672–1685, August 2014
How to Cite
Costa, E. C., Gaspar, V. M., Coutinho, P. and Correia, I. J. (2014), Optimization of liquid overlay technique to formulate heterogenic 3D co-cultures models. Biotechnol. Bioeng., 111: 1672–1685. doi: 10.1002/bit.25210
- Issue published online: 25 JUN 2014
- Article first published online: 25 FEB 2014
- Accepted manuscript online: 6 FEB 2014 05:31AM EST
- Manuscript Accepted: 31 JAN 2014
- Manuscript Revised: 29 JAN 2014
- Manuscript Received: 15 NOV 2013
- Portuguese Foundation for Science and Technology (FCT). Grant Numbers: PTDC/EBB-BIO/114320/2009, PEst-OE/EGE/UI4056/2011, PEst-C/SAU/UI0709/2011
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Figure S1. Representative optical contrast microscopy images processing by ImageJ to determinate spheroids shape and size overtime.
Figure S2. Size analysis of various spheroids produced by LOT.
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