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FilenameFormatSizeDescription
bit25221-sm-0001-SupFig-S1.tif1049KFigure S1. Quantification of the amount of immobilized antibody on gold nanoparticles.
bit25221-sm-0001-SupFig-S2.tif4289KFigure S2. Comparison of plasmon wavelengths for four mAbs with diverse self-association behavior. Plasmon wavelengths for antibody-gold conjugates prepared using (A) mAb A, (B) mAb D, (C) mAb C, and (D) mAb F in the absence and presence of 150 mM NaCl at pH 6 (25 mM histidine).
bit25221-sm-0001-SupFig-S3.tif2207KFigure S3. Impact of mAb concentration on the plasmon shifts for antibody-gold conjugates. A,B: The total antibody concentration (mAb + pAb) used to prepare the (A) mAb B and (B) mAb D conjugates was systematically varied (final concentrations of 16–128 µg/mL), the plasmon wavelengths were measured after incubation for 1 h, and the plasmon shifts were calculated using Equation (1). The final solution conditions were 0 mM NaCl (mAb B) and 150 mM NaCl (mAb D) at pH 6 (25 mM histidine).
bit25221-sm-0001-SupFig-S4.tif2193KFigure S4. Effect of incubation time on the plasmon shifts for antibody-gold conjugates. (A,B) Antibody-gold conjugates for (A) mAb B and (B) mAb D were prepared using 64 μg/mL mAb (final concentration), the plasmon wavelengths were measured after various incubation times (0.5–14 h), and the plasmon shifts were calculated using Equation (1). The final solution conditions were 0 mM NaCl (mAb B) and 150 mM NaCl (mAb D) at pH 6 (25 mM histidine).
bit25221-sm-0001-SupData-S1.docx13KSupporting Information.

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