A method of automatic pH control of a bicarbonate-CO2 buffer system for the submerged culture of hamster kidney cells

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Abstract

The equipment and operational techniques are described which were found suitable to control pH in the range 6.8–7.8 pH, within ±0.03 pH units of the desired value, at cell concentrations up to a maximum of 2.5 × 106/ml. The results of batch growth of a suspension strain of the BHK cell (clone 13) under conditions of controlled pH are given and the significance of these results is discussed.

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