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The influence of dissolved oxygen partial pressure on the level of various enzymes in mouse LS cells

Authors

  • Daphne A. Self,

    1. Biochemical Engineering Section, Department of Chemical Engineering, University College, London, England
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  • D. G. Kilburn,

    1. Biochemical Engineering Section, Department of Chemical Engineering, University College, London, England
    Current affiliation:
    1. Vaccine Department, Rijks Instituut voor de Volksgezondheid, Bilthoven, The Netherlands
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  • M. D. Lilly

    1. Biochemical Engineering Section, Department of Chemical Engineering, University College, London, England
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Abstract

The intracellular levels of seven enzymes in mouse LS cells growing in suspension culture at controlled dissolved oxygen partial pressures (pO2) have been measured. During the growth of each culture large fluctuations were observed in the levels of some enzymes, particularly aldolase and cytochrome oxidase. Mean values for the concentration of each enzyme during the growth phase have been calculated. These results are discussed in relation to previous observations made on the growth of mouse LS cells.

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