Continuous enzymatic saccharification of cellulose with culture filtrates of trichoderma viride QM 6a.


  • Tarun K. Ghose

    1. Microbiology Division, Food Laboratory, U. S. Army Natick Laboratories, Natick, Massachusetts 01760
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    • On leave from Jadavpur University, Calcutta, India.

  • Paper presented at the 3rd International Fermentation Symposium, New Brunswick, New Jersey, September 3–6, 1968.


Continuous saccharification of Solka Floc (cellulose pulp) in single and four-vessel stirred-tank reactor systems has been possible employing enzymes obtained directly from submerged fermentation of Trichoderma viride QM 6a. Studies on the effect of modification of the solid substrate, enzyme stability, substrate concentration, and the influence of reducing sugar concentration on the rate of hydrolysis are reported. While susceptibility of substrate to digestion is not affected by heating alone, it is strikingly increased by heating plus grinding, or by grinding following heating. Batch and steady state continuous saccharification experiments have yielded more than 5% reducing sugar in the effluent with a dilution rate of 0.025 hr−1 at 50°C, at a substrate level of 10%. An average glucose concentration of 3.4% has been obtained in the effluent of a continuous saccharification using 5% substrate at the same dilution rate and temperature.