NAS/NRC Visiting Scientist at the U. S. Army Natick Laboratories.
Kinetic studies on insoluble cellulose–cellulase system
Article first published online: 18 FEB 2004
Copyright © 1975 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 17, Issue 10, pages 1421–1433, October 1975
How to Cite
Huang, A. A. (1975), Kinetic studies on insoluble cellulose–cellulase system. Biotechnol. Bioeng., 17: 1421–1433. doi: 10.1002/bit.260171003
- Issue published online: 18 FEB 2004
- Article first published online: 18 FEB 2004
- Manuscript Accepted: 28 APR 1975
Enzymatic hydrolysis of insoluble amorphous cellulose by Trichoderma viride cellulase was investigated in a batch reactor at several substrate concentrations and three enzyme levels. The reactions were carried out at 50°C and pH 4.8. Enzyme was rapidly adsorbed onto solids on contact, then gradually returned to the liquid phase as the reaction proceeded. A kinetic model that considered the fast adsorption which was followed by the slow reaction, and subsequent product inhibition was developed to interpret the experimental observations. The resulting equation successfully correlated the data for up to 70% conversion. The methods for determining the kinetic parameters are discussed.