The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels

Authors

  • M. Kierstan,

    1. Tate & Lyle Ltd., Group Research and Development, Philip Lyle Memorial Research Laboratory, P.O. Box 68, Reading, Berkshire, RG6 2BX, United Kingdom
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  • C. Bucke

    1. Tate & Lyle Ltd., Group Research and Development, Philip Lyle Memorial Research Laboratory, P.O. Box 68, Reading, Berkshire, RG6 2BX, United Kingdom
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Abstract

Saccharomyces cerevisiae cells, Kluyveromyces marxianus cells, inulase, glucose oxidase, chloroplasts, and mitochondria were immobilized in calcium alginate gels.

Ethanol production from glucose solutions by an immobilized preparation of S. cerevisiae was demonstrated over a total of twenty-three days, and the half-life of such a preparation was shown to be about ten days.

Immobilized K. marxianus, inulase, and glucose oxidase preparations were used to demonstrate the porosity and retraining properties of calcium alginate gels.

Calcium alginate–immobilized chloroplasts were shown to perform the Hill reaction.

Some experiments with immobilized mitochondria are reported.

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