The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels
Article first published online: 18 FEB 2004
Copyright © 1977 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 19, Issue 3, pages 387–397, March 1977
How to Cite
Kierstan, M. and Bucke, C. (1977), The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels. Biotechnol. Bioeng., 19: 387–397. doi: 10.1002/bit.260190309
- Issue published online: 18 FEB 2004
- Article first published online: 18 FEB 2004
- Manuscript Accepted: 10 OCT 1976
Saccharomyces cerevisiae cells, Kluyveromyces marxianus cells, inulase, glucose oxidase, chloroplasts, and mitochondria were immobilized in calcium alginate gels.
Ethanol production from glucose solutions by an immobilized preparation of S. cerevisiae was demonstrated over a total of twenty-three days, and the half-life of such a preparation was shown to be about ten days.
Immobilized K. marxianus, inulase, and glucose oxidase preparations were used to demonstrate the porosity and retraining properties of calcium alginate gels.
Calcium alginate–immobilized chloroplasts were shown to perform the Hill reaction.
Some experiments with immobilized mitochondria are reported.