Computer model for glucose-limited growth of a single cell of Escherichia coli B/r-A

Authors

  • M. M. Domach,

    1. School of Chemical Engineering, Cornell University, Ithaca, New York 14853
    Current affiliation:
    1. Carnegie-Mellon University, Department of Chemical Engineering, Pittsburgh, Pennsylvania 15213
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  • S. K. Leung,

    1. School of Chemical Engineering, Cornell University, Ithaca, New York 14853
    Current affiliation:
    1. Union Carbide Corp., R&D, Charleston, West Virginia 15309m
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  • R. E. Cahn,

    1. School of Chemical Engineering, Cornell University, Ithaca, New York 14853
    Current affiliation:
    1. E.I. duPont de Nemours & Co., Grasselli Plant, R&D Engineering, Linden, New Jersey 07036m
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  • G. G. Cocks,

    1. School of Chemical Engineering, Cornell University, Ithaca, New York 14853
    Current affiliation:
    1. Los Alamos Scientific Laboratory, Mail Stop 981, Los Alamos, New Mexico 87545
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  • M. L. Shuler

    Corresponding author
    1. School of Chemical Engineering, Cornell University, Ithaca, New York 14853
    • School of Chemical Engineering, Cornell University, Ithaca, New York 14853
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Abstract

A computer model is described which is capable of predicting changes in cell composition, cell size, cell shape, and the timing of chromosome synthesis in response to changes in external glucose limitation. The model is constructed primarily from information on unrestricted growth in glucose minimal medium. The ability of the model to make reasonable quantitative predictions under glucose-limitation is a test of the plausibility of the basic biochemical mechanisms included in the model. Such a model should be of use in differentiating among competing hypotheses for biological mechanisms and in suggesting as yet unobserved phenomena. The last two points are illustrated with the testing of a mechanism for the control of the initiation of DNA synthesis and predictions on cellwidth variations during the division cycle.

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