Application of immobilized invertase to continuous hydrolysis of concentrated sucrose solutions
Article first published online: 18 FEB 2004
Copyright © 1984 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 26, Issue 4, pages 347–351, April 1984
How to Cite
Monsan, P. and Combes, D. (1984), Application of immobilized invertase to continuous hydrolysis of concentrated sucrose solutions. Biotechnol. Bioeng., 26: 347–351. doi: 10.1002/bit.260260409
- Issue published online: 18 FEB 2004
- Article first published online: 18 FEB 2004
- Manuscript Accepted: 2 SEP 1983
Invertase immobilized onto corn grits was utilized in the hydrolysis of highly concentrated sucrose solutions producting liquid sugar solutions containing glucose and fructose. Comparisons of conversion efficiencies of this immobilized invertase in a continuous stirredtank reactor and a plug-flow reactor indicated that the plug-flow reactor has an higher efficiency. Continuous sucrose hydrolysis was then performed in 0.1- and 1-L tubular reactors. This tenforld scaling-up was achieved without any noticeable loss in efficiency. This process thus was scaled-up to a 17.6-L pilot reactor set in a cane sugar refinery. This reactor was fed with highly concentrated sucrose solutions [71% (w/w)] to produce invert sugar syrup with the desired inversion degree. It allows a productivity equal to 9.1 kg sucrose hydrolyzed/h in the case of a 69% (w/w) sucrose initial concentration with a 72% conversion rate.