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Article
Determining the inhibition constants in the HCH-1 model of cellulose hydrolysis
Article first published online: 18 FEB 2004
DOI: 10.1002/bit.260260719
Copyright © 1984 John Wiley & Sons, Inc.
1097-0290/asset/cover.gif?v=1&s=169bf64713ffd27abfe496301dbedc7070f98e92 "Biotechnology and Bioengineering")
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How to Cite
Holtzapple, M. T., Caram, H. S. and Humphrey, A. E. (1984), Determining the inhibition constants in the HCH-1 model of cellulose hydrolysis. Biotechnol. Bioeng., 26: 753–757. doi: 10.1002/bit.260260719
Publication History
- Issue published online: 18 FEB 2004
- Article first published online: 18 FEB 2004
- Manuscript Accepted: 27 DEC 1983
- Abstract
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- Cited By
Abstract
The products of cellulose hydrolysis, glucose and cellobiose, caused noncompetitive inhibition patterns to be exhibited when Thermomonospora sp. YX cellulase hydrolyzed dyed cellulose. The glucose binding constant, β1, was 0.00683 ± 0.00031 L/g and the cellobiose binding constant, β2, was 0.095 ± 0.0058 L/g. Thus, cellobiose was about 14 times more inhibitory than glucose.