Effect of crystallinity and degree of polymerization of cellulose on enzymatic saccharification
Article first published online: 18 FEB 2004
Copyright © 1984 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 26, Issue 10, pages 1219–1222, October 1984
How to Cite
Puri, V. P. (1984), Effect of crystallinity and degree of polymerization of cellulose on enzymatic saccharification. Biotechnol. Bioeng., 26: 1219–1222. doi: 10.1002/bit.260261010
- Issue published online: 18 FEB 2004
- Article first published online: 18 FEB 2004
- Manuscript Accepted: 27 FEB 1984
Lignocellulose materials were pretreated by methods known to improve enzymatic saccharification, and the percentage crystallinity (x-ray diffraction) and degree of polymerization were measured. It was observed that although the percentage crystallinity of cellulose was not altered by alkaline explosion (AE), carbon dioxide explosion (CE), ozone, and sodium chlorite treatment, very great increases were obtained in the extent of enzymatic saccharification. All the pretreatments studied except sodium chlorite caused significant reduction in degree of polymerization. It appears likely that the rate and extent of saccharification is governed by particle size, surface area, and degree of polymerization, since crystallinity effects alone do not explain the observed trends in the hydrolysis data.