Preparation and characterization of immobilized growing cells of Zymomonas mobilis for ethanol production

Authors

  • V. K. Jain,

    1. Université de Provence Laboratoire de Chimie Bactérienne, CNRS Marseille Cedex 9, France
    Current affiliation:
    1. Dept. Biochemical Engineering and Food Technology, H.B. Technological Institute, Kanpur, 208002 India
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  • I. Toran-Diaz,

    1. Université de Provence Laboratoire de Chimie Bactérienne, CNRS Marseille Cedex 9, France
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  • J. Barrati

    Corresponding author
    1. Université de Provence Laboratoire de Chimie Bactérienne, CNRS Marseille Cedex 9, France
    • Université de Provence Laboratoire de Chimie Bactérienne, CNRS Marseille Cedex 9, France
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Abstract

Zymomonas mobilis cells were entrapped in K. carrageenan. Growth was observed with the immobilized cell preparation. The kinetic and yield parameters for the conversion of fructose to ethanol were nearly identical to free cells. The same preparation of immobilized cells was used in six repeated batch runs and at the end sixthbatch fructose was converted to ethanol more rapidly and efficiently with ethanol productivity of 14 g/L h and 96% conversion of fructose. The effect of high fructose and ethanol levels on specific fructose uptake rate and ethanol productivity was studied and quantitatively analyzed.

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