Oxygen transfer in animal cell culture medium
Article first published online: 18 FEB 2004
Copyright © 1987 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 30, Issue 3, pages 368–373, 20 August 1987
How to Cite
Lavery, M. and Nienow, A. W. (1987), Oxygen transfer in animal cell culture medium. Biotechnol. Bioeng., 30: 368–373. doi: 10.1002/bit.260300307
- Issue published online: 18 FEB 2004
- Article first published online: 18 FEB 2004
- Manuscript Accepted: 13 AUG 1986
Both kLa and kL measurements were carried out by an unsteady state technique at impeller speeds ranging from 1.6 to 5.8 s−1 in a mechanically agitated animal cell culture vessel of working volume 1.5 L. Checks were made that the time constant of the oxygen electrode was negligible compared to the time for aeration and that the oxygen electrode reading was not a function of agitator speed in the range employed. The kL values by surface aeration of (1.18–3.54) × 10−5 m/s and kLa values by sparged aeration of (2.8–8.5) × 10−4 s−1 were found. The former are in reasonable agreement with published experimental values and the latter in accord with values estimated from published correlations based on agitator power input and aeration rate. The fluids used were water, basal medium, and basal medium supplemented with 5% (v/v) foetal calf serum; for each of these, kL and kLa values were similar. However, the addition of silicone antifoam (6 PPM) reduced the kLa value by ca. 50%.