A flow injection analysis system for fermentation monitoring and control
Article first published online: 18 FEB 2004
Copyright © 1989 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 34, Issue 4, pages 423–428, 5 August 1989
How to Cite
Garn, M., Gisin, M., Thommen, C. and Cevey, P. (1989), A flow injection analysis system for fermentation monitoring and control. Biotechnol. Bioeng., 34: 423–428. doi: 10.1002/bit.260340402
- Issue published online: 18 FEB 2004
- Article first published online: 18 FEB 2004
An automated analysis system for on-line fermentation monitoring is presented. The modular system consists of an in-line sterilizeable crossflow microfilter, a selection valve that allows injection of sample or standards, a degassing unit, a dilution module, and a FIA manifold with a spectrophotometric UV/VIS detector. In the dilution module samples are conditioned and diluted depending upon concentration of analyte and the working range of the analyzer. Methods for the monitoring of glucose, ethanol, ammonia and phosphate are described. Results from the monitoring of glucose and their use in fermentation control are presented. The maximal analysis frequency is 30 samples per hour including the dilution of 1 : 200. Detection limits are 5 mg/L for ethanol and glucose, 1 mg/L for phosphate and 50 mg/L for ammonia.