Inhibition of Trichoderma reesei cellulase by sugars and solvents
Article first published online: 18 FEB 2004
Copyright © 1990 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 36, Issue 3, pages 275–287, July 1990
How to Cite
Holtzapple, M., Cognata, M., Shu, Y. and Hendrickson, C. (1990), Inhibition of Trichoderma reesei cellulase by sugars and solvents. Biotechnol. Bioeng., 36: 275–287. doi: 10.1002/bit.260360310
- Issue published online: 18 FEB 2004
- Article first published online: 18 FEB 2004
- Manuscript Accepted: 28 DEC 1989
Inhibition of Trichoderma reesei cellulase by sugars (glucose, δ-gluconolactone, and cellobiose) and solvents (ethanol, butanol, and acetone) was studied using cellulose azure. Glucose, cellobiose, ethanol, and butanol were noncompetitive inhibitors, δ-gluconolactone was a mixed inhibitor, and acetone was a noncompetitive activator. Converting cellobiose to glucose reduces the effective inhibitor binding constant by 6 times and converting cellobiose to ethanol reduces it by 16 times.