Communications to the Editor
Measuring kLa with randomly pulsed dynamic method
Article first published online: 18 FEB 2004
Copyright © 1991 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 37, Issue 9, pages 889–893, 15 April 1991
How to Cite
Gauthier, L., Thibault, J. and LeDuy, A. (1991), Measuring kLa with randomly pulsed dynamic method. Biotechnol. Bioeng., 37: 889–893. doi: 10.1002/bit.260370914
- Issue published online: 18 FEB 2004
- Article first published online: 18 FEB 2004
- Manuscript Accepted: 23 OCT 1990
- Manuscript Received: 26 APR 1990
- oxygen transfer coefficient;
- pulsed dynamic method;
- mechanically agitated vessels
This reports on the determination of the overall oxygen transfer coefficient in a mechanically agitated vessel using a randomly pulsed dynamic method. This method consists in exciting the system by randomly switching the inlet gas stream with air or nitrogen with an identical volumetric flow rate. A pseudo-random binary sequence was used. This procedure is routinely used in process control for the identification of system's transfer function. The pulsed dynamic method gives good reliability (as compared with the traditional gassing-out method) and reproducibility in water. However, further improvement is needed before it can be used to monitor on-line the kLa during a fermentation.