Critical assessment of gassing-in methods for measuring kla in fermentors
Article first published online: 19 FEB 2004
Copyright © 1991 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 38, Issue 4, pages 323–330, 5 August 1991
How to Cite
Linek, V., Sinkule, J. and Beneš, P. (1991), Critical assessment of gassing-in methods for measuring kla in fermentors. Biotechnol. Bioeng., 38: 323–330. doi: 10.1002/bit.260380402
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 11 DEC 1990
- Manuscript Received: 29 JUN 1990
- oxygen transfer;
- oxygen-enriched air;
- dynamic pressure method;
- steady-state feeding method
The reliability of dynamic measurement methods of kla in fermentors using a step oxygen concentration change in the feed gas was tested. The tests were performed both for the original variant using the nitrogen ⇌ air exchange and the newly presented variant using the oxygen-enriched air (27 vol % O2) → air exchange. The testing consisted in comparing kla values determined from these methods with values determined from the steady-state Na2SO3 feeding method and the dynamic pressure method, the reliability of which was proven earlier. The measurements were done in water (coalescent batch) and in 0.5M Na2SO4 solution with and without the addition of 1 wt % carboxymethylcellulose (noncoalescent batches). It was found that in noncoalescent liquids the methods tested give extremely low kla values (as low as 15% of the correct value). The methods are defective in principle irrespective of the gases used for exchange.