Communications to the Editor
Large-Scale, High-density freezing of hybridomas and its application to high-density culture
Article first published online: 19 FEB 2004
Copyright © 1991 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 38, Issue 9, pages 1110–1113, November 1991
How to Cite
Ninomiya, N., Shirahata, S., Murakami, H. and Sugahara, T. (1991), Large-Scale, High-density freezing of hybridomas and its application to high-density culture. Biotechnol. Bioeng., 38: 1110–1113. doi: 10.1002/bit.260380920
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 22 MAR 1991
- Manuscript Received: 6 NOV 1990
- high-density freezing;
- high-density culture;
Large-scale, high-density freezing of hybridomas was studied to apply frozen cells to start high-density culture. We showed here that hybridomas can be frozen at 1.5 × 108 cells/mL, without decrement in viability and proliferating activity. Blood transporting bags were used for large-scale freezing to store 25 mL of cell suspension with a cell density, 1.5 × 108/mL. The number of cells stored in a bag (3.0 × 109 cells) was enough to start a high-density culture at a 10 times higher cell density (6.0 × 106 cells/mL) than normal inoculation, and the cells proliferated to 107 cells/mL within 2 days. These results indicate that the large-scale freezing method is useful for large-scale culture of mammalian cells.