Saccharification of steam-exploded poplar wood
Article first published online: 19 FEB 2004
Copyright © 1991 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 38, Issue 11, pages 1308–1317, 20 December 1991
How to Cite
Excoffier, G., Toussaint, B. and Vignon, M. R. (1991), Saccharification of steam-exploded poplar wood. Biotechnol. Bioeng., 38: 1308–1317. doi: 10.1002/bit.260381108
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 26 APR 1991
- Manuscript Received: 4 OCT 1990
- Trichoderma reesei CL-847;
- steam explosion treatment;
Effects of time, temperature, and pH during the steam explosion of poplar wood were studied with the aim of optimize both pentoses recovery and enzymatic hydrolysis efficiency. Steam explosion of acid impregnated wood chips allowed the recovery of 70% of potential xylose as monomers (217°C, 120 s) Enzymatic hydrolysis of pretreated fiber with Trichoderma reesei CL-847 cellulase system increased progressively with the severity of the steam treatment conditions. The best yield in term of glucose recovery after 24 h of enzymatic hydrolysis was 70% of potential glucose (225°C, 120 s). Deactivation by adsorption on lignin of Trichoderma reesei cellulases and inhibition of these enzymes by low-molecular-weight phenols and trihydroxybutyric acids were noticed.