Communications to the Editor
Correlating the effect of pretreatment on the enzymatic hydrolysis of straw
Article first published online: 19 FEB 2004
Copyright © 1992 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 39, Issue 1, pages 113–116, 5 January 1992
How to Cite
Koullas, D. P., Christakopoulos, P., Kekos, D., Macris, B. J. and Koukios, E. G. (1992), Correlating the effect of pretreatment on the enzymatic hydrolysis of straw. Biotechnol. Bioeng., 39: 113–116. doi: 10.1002/bit.260390116
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 22 MAY 1991
- Manuscript Received: 8 JAN 1991
- enzymic hydrolysis models;
- pretreated lignocellulosics hydrolysis;
- Fusarium oxysporum cellulases
Avicell, Alkali-treated straw cellulose (ATSC), and wheat straw were ball-milled to reduce crystallinity; wheat straw was delignified by hot (120°C) sodium hydroxide solutions of various concentrations. The physically and chemically pretreated cellulosic materials were hydrolyzed by the cellulases of Fusarium oxysporum strain F3. Enzymic hydrolysis data were fitted by the hyperbolic correlation of Holtzapple, which involves two kinetic parameters, the maximum conversion (Xmax), and the enzymic hydrolysis time corresponding to 50% of Xmax (t1/2). An empirical correlation between Xmax and cellulose crystallinity, lignin content, and degree of delignification has been found under our experimental conditions. Complete cellulose hydrolysis is shown to be possible at less than 60% crystallinity indices or less than 10% lignin content.