Hydrolysis of penicillin G by combination of immobilized penicillin acylase and electrodialysis
Article first published online: 19 FEB 2004
Copyright © 1992 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 39, Issue 2, pages 171–175, 20 January 1992
How to Cite
Ishimura, F. and Suga, K.-I. (1992), Hydrolysis of penicillin G by combination of immobilized penicillin acylase and electrodialysis. Biotechnol. Bioeng., 39: 171–175. doi: 10.1002/bit.260390208
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 26 JUN 1991
- Manuscript Received: 15 JAN 1991
- penicillin acylase;
- penicillin G;
- product inhibition
Phenylacetic acid, as inhibitory product, was formed from a hydrolysis of penicillin G by immobilized penicillin acylase. In this article, electrodialysis was applied to remove phenylacetic acid continuously from the reaction mixture and to enhance an efficiency of the reaction. When 268 and 537 mM of penicillin G solution were used as the substrate, the concentration of phenylacetic acid in the reaction mixture could be maintained at less than 81 and 126 mM, respectively, and eventually, 86% and 88% of phenylacetic acid produced were removed from the reaction mixture at the end of the hydrolysis, respectively. Times required to reach 96% and 94.8% conversion from 268 and 537 mM of initial penicillin G could be reduced to 65% and 64% respectively, by means of electrodialysis; while 3.0% and 4.3% of initial penicillin G of 268 and 537 mM were permeated out of the reaction chamber during the hydrolysis, respectively. However, a loss of penicillin G by permeation could be reduced from 4.3% to 3.4% by a repeated addition of penicillin G.