Quantification of cell culture factors affecting recombinant protein yields in baculovirus-infected insect cells
Article first published online: 19 FEB 2004
Copyright © 1992 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 39, Issue 6, pages 614–618, 15 March 1992
How to Cite
Lindsay, D. A. and Betenbaugh, M. J. (1992), Quantification of cell culture factors affecting recombinant protein yields in baculovirus-infected insect cells. Biotechnol. Bioeng., 39: 614–618. doi: 10.1002/bit.260390605
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 19 SEP 1991
- Manuscript Received: 14 JUN 1991
- insect cell;
- recombinant DNA
An experimental study was undertaken to quantify the effects of infection cell density, medium condition, and surface aeration on recombinant protein yields in insect cells. In the absence of surface aeration and fresh medium, insect cells generated higher product yields (on a per cell basis) when infected with recombinant baculovirus at low cell densities, LCD (3 × 105−4 × 105 cells/mL), than at high cell densities, HCD (>0.9 × 106 cells/mL), for two distinct baculovirus types. Surface aeration of a HCD culture infected in spent medium improved β-glactosidase yields 5-fold over the nonaerated case. Surface aeration and medium replenishment improved β-galactosidase yields of a HCD culture by 20-fold (compared to a 1.6-fold improvement for a LCD culture), resulting in cultures with productivties that were independent of the cell density at infection.