Effect of temperature on hybridoma cell cycle and MAb production
Article first published online: 19 FEB 2004
Copyright © 1992 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 40, Issue 3, pages 427–431, July 1992
How to Cite
Bloemkolk, J.-W., Gray, M. R., Merchant, F. and Mosmann, T. R. (1992), Effect of temperature on hybridoma cell cycle and MAb production. Biotechnol. Bioeng., 40: 427–431. doi: 10.1002/bit.260400312
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 28 FEB 1992
- Manuscript Received: 16 OCT 1991
- cell cycle;
- monoclonal antibody;
The kinetics of growth and antibody formation of an anti–interleukin-2 producing hybridoma line were studied in suspension culture at temperatures ranging from 34°C to 39°C. Flow cytometry was used to determine the effect of temperature on the cell cycle. Maximum cell density and monoclonal antibody yield were observed at 37°C. The specific monoclonal antibody production rate was approximately constant throughout each batch experiment. Lower temperatures caused cells to stay longer in the G1-phase of the cell cycle, but temperature had only a marginal effect on the specific antibody production rate. Arresting of cells in the G1-phase by means of temperature was, therefore, not suited for enhanced monoclonal antibody production. Rather, antibody production for this hybridoma was directly linked to viable cell concentration. © 1992 John Wiley & Sons, Inc.