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Keywords:

  • hybridoma growth;
  • turbidity sensor;
  • cell concentration monitoring;
  • bioprocess control;
  • growth rate

Abstract

A high-sensitivity turbidity probe was used for on-line monitoring of the cell concentration in batch hybridoma cultivation. Good correlation between off-line cell counts and the linearized sensor signal was found. The quality of the signal was sufficiently high to provide for on-line estimation of the specific growth rate using an efficient filtering procedure. These positive results suggest that such laser turbidity sensors will facilitate development of systems for on-line monitoring and control of animal cell cultivations. © 1992 John Wiley & Sons, Inc.