Recombinant trypsin production in high cell density fed-batch cultures in Escherichia coli
Article first published online: 19 FEB 2004
Copyright © 1993 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 41, Issue 8, pages 781–790, 5 April 1993
How to Cite
Yee, L. and Blanch, H. W. (1993), Recombinant trypsin production in high cell density fed-batch cultures in Escherichia coli. Biotechnol. Bioeng., 41: 781–790. doi: 10.1002/bit.260410804
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 12 NOV 1992
- Manuscript Received: 8 JUN 1992
- Escherichia coli;
- fed batch;
- high cell density;
Fed-batch techniques were employed to obtain high cell density cultures (92–100 g DCW/L) of Escherichia coli strain X90 producing a recombinant serine protease, rat anionic trypsin, secreted to the periplasm. The specific growth rate was controlled to minimize growth-inhibiting acetate formation by utilizing an exponential feeding profile determined from mass balance equation. The volumetric yield of recombinant rat anionic trypsin was 56 mg/L, and the final cell density was 92 g DCW/L when the culture was induced in the late logarithmic phase. However, when the culture was induced in the early logarithmic phase, the volumetric yield was 13 mg/L and the final cell density was 14 g DCW/L. Thus, the induction timing is shown to have a significant effect on the final cell density as well as the overall volumetric yield of the recombinant protease. © 1993 Wiley & Sons, Inc.