Cellulose morphology and enzymatic reactivity: A modified solute exclusion technique
Article first published online: 19 FEB 2004
Copyright © 1994 John Wiley & Sons, Inc.
Biotechnology and Bioengineering
Volume 43, Issue 5, pages 381–387, 5 March 1994
How to Cite
Gama, F. M., Teixeira, J. A. and Mota, M. (1994), Cellulose morphology and enzymatic reactivity: A modified solute exclusion technique. Biotechnol. Bioeng., 43: 381–387. doi: 10.1002/bit.260430506
- Issue published online: 19 FEB 2004
- Article first published online: 19 FEB 2004
- Manuscript Accepted: 12 OCT 1993
- Manuscript Received: 3 MAR 1993
- cellulose morphology;
- surface area
An expeditious and accurate simplification of Stone and Scallan's solute exclusion technique was developed, thereby avoiding several sources of experimental error coupled with the determination of cellulose pore volume. Using this method, it is shown that cellulolytic enzymes do not enter into the micropores of five studied celluloses. These results suggestes that hydrolysis occurs initially at the external surface of the fibers. This surface area was calculated with the help of adsorption isotherms of bovine serum albumin. The obtained values for the different samples agree with the microscopically observed cellulose morphology. The correlation obtained by several authors relating cellulose porosity and its digestibility is explained as a consequence of the lower crystallinity and easier fragmentation of the more porous celluloses during hydrolysis. © 1994 John Wiley & Sons, Inc.